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Corning Life Sciences modified boyden chamber
Modified Boyden Chamber, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+boyden+chambers/modified+boyden+chamber/pm40483310-299-29-34
Average 90 stars, based on 1 article reviews
modified boyden chamber - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Transfection:

Article Title: Curcumin attenuates lncRNA H19-induced epithelial-mesenchymal transition in tamoxifen-resistant breast cancer cells
Article Snippet: .. Following transfection and curcumin treatment, cell invasion was assessed using modified Boyden chambers (pore size, 8.0 μm; Costar; Corning, Inc.). ..

Modification:

Article Title: Curcumin attenuates lncRNA H19-induced epithelial-mesenchymal transition in tamoxifen-resistant breast cancer cells
Article Snippet: .. Following transfection and curcumin treatment, cell invasion was assessed using modified Boyden chambers (pore size, 8.0 μm; Costar; Corning, Inc.). ..

Article Title: Id2 exerts tumor suppressor properties in lung cancer through its effects on cancer cell invasion and migration.
Article Snippet: .. A Transwell assay with Matrigel-coated transwell filters (BD Biosciences, Franklin Lakes, NJ, USA) and modified Boyden chambers (pore size: 8 mm; Corning Costar, Cambridge, MA, USA) was used to assess cell invasiveness as previously described (29). .. After 18 h of incubation at 37°C, the cells were fixed for 10 min in methanol (Sigma-Aldrich) and then stained at room temperature for 30 min with 10% Gemisa Stain solution (Sigma-Aldrich).

Article Title: Crosstalk of human coronary perivascular adipose-derived stem cells with vascular cells: role of tissue factor.
Article Snippet: The coronary perivascular adipose tissue (cPVAT) has been associated to the burden of cardiovascular risk factors and to the underlying vessel atherosclerotic plaque severity.. Although the “outside to inside” hypothesis of PVAT-derived-adipokine regulation of vessel function is currently accepted, whether the resident mesenchymal stem cells (ASCs) in PVAT have a regulatory role on the underlying vascular arterial smooth muscle cells (VSMCs) is not known.. Here, we investigated the interactions between resident PVAT-ASCs and VSMCs.

Article Title: Nrf1 is an indispensable redox-determining factor for mitochondrial homeostasis by integrating multi-hierarchical regulatory networks
Article Snippet: The results were analyzed by the FlowJo 7.6.1 software (FlowJo, Ashland, OR, USA) before being presented. .. The Transwell-based migration and invasion assays were conducted in the modified Boyden chambers (Transwell; Corning Inc. Lowell, MA, USA). ..

Article Title: ADAM28 overexpression regulated via the PI3K/Akt pathway is associated with relapse in de novo adult B-cell acute lymphoblastic leukemia.
Article Snippet: B-cell acute lymphoblastic leukemia (B-ALL) in adults is a very challenging disease.. Relapse following remission after induction chemotherapy remains the major barrier to patient survival.. ADAM28 is overexpressed in several human tumors and is related to cell proliferation and lymph node metastasis.

Article Title: CircZFR functions as a sponge of miR-578 to promote breast cancer progression by regulating HIF1A expression
Article Snippet: .. Cell migration and invasion were detected by the transwell assay using modified Boyden chambers in 24-transwell plates (8 μm pores, Corning, Amsterdam, The Netherlands). .. Chambers of cell invasion assays consisted of Matrigel-precoated membrane inserts (Corning).

Article Title: Id2 exerts tumor suppressor properties in lung cancer through its effects on cancer cell invasion and migration
Article Snippet: .. A Transwell assay with Matrigel-coated transwell filters (BD Biosciences, Franklin Lakes, NJ, USA) and modified Boyden chambers (pore size: 8 μm; Corning Costar, Cambridge, MA, USA) was used to assess cell invasiveness as previously described ( ). .. After 18 h of incubation at 37°C, the cells were fixed for 10 min in methanol (Sigma-Aldrich) and then stained at room temperature for 30 min with 10% Gemisa Stain solution (Sigma-Aldrich).

Pore Size:

Article Title: Curcumin attenuates lncRNA H19-induced epithelial-mesenchymal transition in tamoxifen-resistant breast cancer cells
Article Snippet: .. Following transfection and curcumin treatment, cell invasion was assessed using modified Boyden chambers (pore size, 8.0 μm; Costar; Corning, Inc.). ..

Article Title: Id2 exerts tumor suppressor properties in lung cancer through its effects on cancer cell invasion and migration.
Article Snippet: .. A Transwell assay with Matrigel-coated transwell filters (BD Biosciences, Franklin Lakes, NJ, USA) and modified Boyden chambers (pore size: 8 mm; Corning Costar, Cambridge, MA, USA) was used to assess cell invasiveness as previously described (29). .. After 18 h of incubation at 37°C, the cells were fixed for 10 min in methanol (Sigma-Aldrich) and then stained at room temperature for 30 min with 10% Gemisa Stain solution (Sigma-Aldrich).

Article Title: Id2 exerts tumor suppressor properties in lung cancer through its effects on cancer cell invasion and migration
Article Snippet: .. A Transwell assay with Matrigel-coated transwell filters (BD Biosciences, Franklin Lakes, NJ, USA) and modified Boyden chambers (pore size: 8 μm; Corning Costar, Cambridge, MA, USA) was used to assess cell invasiveness as previously described ( ). .. After 18 h of incubation at 37°C, the cells were fixed for 10 min in methanol (Sigma-Aldrich) and then stained at room temperature for 30 min with 10% Gemisa Stain solution (Sigma-Aldrich).

Transwell Assay:

Article Title: Id2 exerts tumor suppressor properties in lung cancer through its effects on cancer cell invasion and migration.
Article Snippet: .. A Transwell assay with Matrigel-coated transwell filters (BD Biosciences, Franklin Lakes, NJ, USA) and modified Boyden chambers (pore size: 8 mm; Corning Costar, Cambridge, MA, USA) was used to assess cell invasiveness as previously described (29). .. After 18 h of incubation at 37°C, the cells were fixed for 10 min in methanol (Sigma-Aldrich) and then stained at room temperature for 30 min with 10% Gemisa Stain solution (Sigma-Aldrich).

Article Title: CircZFR functions as a sponge of miR-578 to promote breast cancer progression by regulating HIF1A expression
Article Snippet: .. Cell migration and invasion were detected by the transwell assay using modified Boyden chambers in 24-transwell plates (8 μm pores, Corning, Amsterdam, The Netherlands). .. Chambers of cell invasion assays consisted of Matrigel-precoated membrane inserts (Corning).

Article Title: Id2 exerts tumor suppressor properties in lung cancer through its effects on cancer cell invasion and migration
Article Snippet: .. A Transwell assay with Matrigel-coated transwell filters (BD Biosciences, Franklin Lakes, NJ, USA) and modified Boyden chambers (pore size: 8 μm; Corning Costar, Cambridge, MA, USA) was used to assess cell invasiveness as previously described ( ). .. After 18 h of incubation at 37°C, the cells were fixed for 10 min in methanol (Sigma-Aldrich) and then stained at room temperature for 30 min with 10% Gemisa Stain solution (Sigma-Aldrich).

Migration:

Article Title: Nrf1 is an indispensable redox-determining factor for mitochondrial homeostasis by integrating multi-hierarchical regulatory networks
Article Snippet: The results were analyzed by the FlowJo 7.6.1 software (FlowJo, Ashland, OR, USA) before being presented. .. The Transwell-based migration and invasion assays were conducted in the modified Boyden chambers (Transwell; Corning Inc. Lowell, MA, USA). ..

Article Title: ADAM28 overexpression regulated via the PI3K/Akt pathway is associated with relapse in de novo adult B-cell acute lymphoblastic leukemia.
Article Snippet: B-cell acute lymphoblastic leukemia (B-ALL) in adults is a very challenging disease.. Relapse following remission after induction chemotherapy remains the major barrier to patient survival.. ADAM28 is overexpressed in several human tumors and is related to cell proliferation and lymph node metastasis.

Article Title: CircZFR functions as a sponge of miR-578 to promote breast cancer progression by regulating HIF1A expression
Article Snippet: .. Cell migration and invasion were detected by the transwell assay using modified Boyden chambers in 24-transwell plates (8 μm pores, Corning, Amsterdam, The Netherlands). .. Chambers of cell invasion assays consisted of Matrigel-precoated membrane inserts (Corning).

Article Title: Tumor- and host-derived heparanase-2 (Hpa2) attenuates tumorigenicity: role of Hpa2 in macrophage polarization and BRD7 nuclear localization.
Article Snippet: Once the bioparticles were engulfed by phagocytosis and entered the acidic phagosome, a substantial increase in red fluorescence was observed and monitored by quantitative live cell imaging (IncuCyte ZOOM Live Cell Analysis System; Essen) [26]. .. Cell migration, spheroids formation, and cytotoxicity Migration assay was performed using modified Boyden chambers (6.5 mm in diameter, 8 μm pore size; Corning, Corning, NY), coated with fibronectin (30 μl; 10 μg/ml), essentially as described [22]. .. Cytotoxicity was estimated by LDH assay performed according to the manufacturer’s (BioLegend) instructions.

Cell Migration Assay:

Article Title: ADAM28 overexpression regulated via the PI3K/Akt pathway is associated with relapse in de novo adult B-cell acute lymphoblastic leukemia.
Article Snippet: B-cell acute lymphoblastic leukemia (B-ALL) in adults is a very challenging disease.. Relapse following remission after induction chemotherapy remains the major barrier to patient survival.. ADAM28 is overexpressed in several human tumors and is related to cell proliferation and lymph node metastasis.



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CtBP2 favors cell migration. A Representative images of K7M2 cell migration after 10 h incubation, as assessed by <t>Boyden</t> <t>chamber</t> assay. CtBP2-silenced K7M2 cells were stained in green, and CtBP2-overexpressing cells were stained in red before seeding (bar = 100 µm). B Estimation plot representing the relative number of migrating cells. The difference between the group means is represented on the right. C Representative images of cell migration (wound healing assay) taken at time 18 h after the wound. Control and CtBP2-silenced K7M2 cells were incubated in the presence or absence of recombinant CYR61 (1 µg/mL). The dotted rectangles outline the initial wound surface. D Quantitative evaluation of the cell migration rate. Results are expressed as box plot ( n = 15–22). An asterisk (*) indicates a statistically significant difference ( p < 0.05 vs. Control)
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CtBP2 favors cell migration. A Representative images of K7M2 cell migration after 10 h incubation, as assessed by <t>Boyden</t> <t>chamber</t> assay. CtBP2-silenced K7M2 cells were stained in green, and CtBP2-overexpressing cells were stained in red before seeding (bar = 100 µm). B Estimation plot representing the relative number of migrating cells. The difference between the group means is represented on the right. C Representative images of cell migration (wound healing assay) taken at time 18 h after the wound. Control and CtBP2-silenced K7M2 cells were incubated in the presence or absence of recombinant CYR61 (1 µg/mL). The dotted rectangles outline the initial wound surface. D Quantitative evaluation of the cell migration rate. Results are expressed as box plot ( n = 15–22). An asterisk (*) indicates a statistically significant difference ( p < 0.05 vs. Control)
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Image Search Results


CtBP2 favors cell migration. A Representative images of K7M2 cell migration after 10 h incubation, as assessed by Boyden chamber assay. CtBP2-silenced K7M2 cells were stained in green, and CtBP2-overexpressing cells were stained in red before seeding (bar = 100 µm). B Estimation plot representing the relative number of migrating cells. The difference between the group means is represented on the right. C Representative images of cell migration (wound healing assay) taken at time 18 h after the wound. Control and CtBP2-silenced K7M2 cells were incubated in the presence or absence of recombinant CYR61 (1 µg/mL). The dotted rectangles outline the initial wound surface. D Quantitative evaluation of the cell migration rate. Results are expressed as box plot ( n = 15–22). An asterisk (*) indicates a statistically significant difference ( p < 0.05 vs. Control)

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: C-terminal binding protein-2 triggers CYR61-induced metastatic dissemination of osteosarcoma in a non-hypoxic microenvironment

doi: 10.1186/s13046-025-03350-6

Figure Lengend Snippet: CtBP2 favors cell migration. A Representative images of K7M2 cell migration after 10 h incubation, as assessed by Boyden chamber assay. CtBP2-silenced K7M2 cells were stained in green, and CtBP2-overexpressing cells were stained in red before seeding (bar = 100 µm). B Estimation plot representing the relative number of migrating cells. The difference between the group means is represented on the right. C Representative images of cell migration (wound healing assay) taken at time 18 h after the wound. Control and CtBP2-silenced K7M2 cells were incubated in the presence or absence of recombinant CYR61 (1 µg/mL). The dotted rectangles outline the initial wound surface. D Quantitative evaluation of the cell migration rate. Results are expressed as box plot ( n = 15–22). An asterisk (*) indicates a statistically significant difference ( p < 0.05 vs. Control)

Article Snippet: The cell migration rate was evaluated using modified Boyden chamber (8 μm pore size Falcon® Transwell inserts, Corning, Boulogne-Billancourt, France).

Techniques: Migration, Incubation, Boyden Chamber Assay, Staining, Wound Healing Assay, Control, Recombinant